Journal: Diagnostics

Article Title: Anti-SARS-CoV-2 IgM Antibody Levels Measured by an In-House ELISA in a Convalescent Latin Population Persist over Time and Exhibit Neutralizing Capacity to Several Variants of Concern

doi: 10.3390/diagnostics14192209

Figure Lengend Snippet: Agreement between the results obtained by the in-house CovIgM-ELISA and the surrogate neutralization assay (cPass).

Article Snippet: Having previously demonstrated that the results provided by the surrogate viral neutralization test (cPass TM GenScript sVNT, Piscataway NJ, USA) [ ] correlate perfectly with the traditional PRNT [ ], a cPass neutralization antibody test was used to determine the levels of neutralizing antibodies in the study population against the wild-type strain and the variants of concern (VOCs), Alpha, Delta and Omicron, which had a wide circulation and prevalence in the Puerto Rican population [ ]. cPass utilizes the recombinant RBD of the SARS-CoV-2 spike protein to detect antibodies that block the RBD from binding to the human ACE2 receptor.

Techniques: Neutralization

Journal: Diagnostics

Article Title: Anti-SARS-CoV-2 IgM Antibody Levels Measured by an In-House ELISA in a Convalescent Latin Population Persist over Time and Exhibit Neutralizing Capacity to Several Variants of Concern

doi: 10.3390/diagnostics14192209

Figure Lengend Snippet: Pearson (r) correlation between the neutralization percentages (sVNT%) and levels of anti-SARS-CoV-2 IgM . The levels of anti-SARS-CoV-2 IgM expressed as average OD 450nm for each sample determined by the in-house CovIgM-ELISA was associated with the neutralization assay (cPass, Genscript) against the wild-type strain and the variants of concern Alpha, Delta and Omicron. Dashed lines on x-axis represent the positive cut-off value (OD > 0.26) for in-house CovIgM-ELISA and the dashed line on the y-axis represent the positive cut-off (sVNT% ≥ 30%) for the neutralization assay. There was significant correlation (* p = 0.02225) between sVNT% and anti-SARS-CoV-2 IgM levels for the wild-type strain.

Article Snippet: Having previously demonstrated that the results provided by the surrogate viral neutralization test (cPass TM GenScript sVNT, Piscataway NJ, USA) [ ] correlate perfectly with the traditional PRNT [ ], a cPass neutralization antibody test was used to determine the levels of neutralizing antibodies in the study population against the wild-type strain and the variants of concern (VOCs), Alpha, Delta and Omicron, which had a wide circulation and prevalence in the Puerto Rican population [ ]. cPass utilizes the recombinant RBD of the SARS-CoV-2 spike protein to detect antibodies that block the RBD from binding to the human ACE2 receptor.

Techniques: Neutralization, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Estimating the Neutralizing Effect and Titer Correlation of Semi-Quantitative Anti-SARS-CoV-2 Antibody Immunoassays

doi: 10.3389/fcimb.2022.822599

Figure Lengend Snippet: Analytical performance of each kit in discriminating SARS-CoV-2 infection.

Article Snippet: To detect neutralizing antibodies using an immunoassay method, the cPass sVNT kit (GenScript, Piscataway, NJ, USA) was used. sVNT measures the inhibition of interactions between the horseradish peroxidase (HRP)-conjugated SARS-CoV-2 spike protein receptor-binding domain (RBD) and the extracellular domain of the human angiotensin-converting enzyme 2 (hACE2) receptor ( ).

Techniques: Infection

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Estimating the Neutralizing Effect and Titer Correlation of Semi-Quantitative Anti-SARS-CoV-2 Antibody Immunoassays

doi: 10.3389/fcimb.2022.822599

Figure Lengend Snippet: Analytical performance for representativeness of neutralizing activity using the pre-defined cut-off values of each immunoassay kit.

Article Snippet: To detect neutralizing antibodies using an immunoassay method, the cPass sVNT kit (GenScript, Piscataway, NJ, USA) was used. sVNT measures the inhibition of interactions between the horseradish peroxidase (HRP)-conjugated SARS-CoV-2 spike protein receptor-binding domain (RBD) and the extracellular domain of the human angiotensin-converting enzyme 2 (hACE2) receptor ( ).

Techniques: Activity Assay

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Estimating the Neutralizing Effect and Titer Correlation of Semi-Quantitative Anti-SARS-CoV-2 Antibody Immunoassays

doi: 10.3389/fcimb.2022.822599

Figure Lengend Snippet: Titer correlation of the analytical performance of the prediction of neutralizing activity using newly calculated cut-off values determined using Youden’s index.

Article Snippet: To detect neutralizing antibodies using an immunoassay method, the cPass sVNT kit (GenScript, Piscataway, NJ, USA) was used. sVNT measures the inhibition of interactions between the horseradish peroxidase (HRP)-conjugated SARS-CoV-2 spike protein receptor-binding domain (RBD) and the extracellular domain of the human angiotensin-converting enzyme 2 (hACE2) receptor ( ).

Techniques: Activity Assay

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Estimating the Neutralizing Effect and Titer Correlation of Semi-Quantitative Anti-SARS-CoV-2 Antibody Immunoassays

doi: 10.3389/fcimb.2022.822599

Figure Lengend Snippet: Serial kinetics of antibody titers measured with each method: (A) PRNT ND50, (B.) GenScript cPass sVNT, (C) Roche Elecsys Anti-SARS-CoV-2, (D) Roche Elecsys Anti-SARS-CoV2 S, and (E) Abbott AdviseDx SARS-CoV2 IgG II.

Article Snippet: To detect neutralizing antibodies using an immunoassay method, the cPass sVNT kit (GenScript, Piscataway, NJ, USA) was used. sVNT measures the inhibition of interactions between the horseradish peroxidase (HRP)-conjugated SARS-CoV-2 spike protein receptor-binding domain (RBD) and the extracellular domain of the human angiotensin-converting enzyme 2 (hACE2) receptor ( ).

Techniques:

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Estimating the Neutralizing Effect and Titer Correlation of Semi-Quantitative Anti-SARS-CoV-2 Antibody Immunoassays

doi: 10.3389/fcimb.2022.822599

Figure Lengend Snippet: Antibody titers by timeline.

Article Snippet: To detect neutralizing antibodies using an immunoassay method, the cPass sVNT kit (GenScript, Piscataway, NJ, USA) was used. sVNT measures the inhibition of interactions between the horseradish peroxidase (HRP)-conjugated SARS-CoV-2 spike protein receptor-binding domain (RBD) and the extracellular domain of the human angiotensin-converting enzyme 2 (hACE2) receptor ( ).

Techniques:

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Estimating the Neutralizing Effect and Titer Correlation of Semi-Quantitative Anti-SARS-CoV-2 Antibody Immunoassays

doi: 10.3389/fcimb.2022.822599

Figure Lengend Snippet: Scatter plot and Pearson’s correlation for each method grouped with acute/convalescent phase. (A) GenScript cPass sVNT, (B) Roche Elecsys Anti-SARS-CoV-2, (C) Roche Elecsys Anti-SARS-CoV2 S, and (D) Abbott AdviseDx SARS-CoV2 IgG II were compared with PRNT, respectively. Each colored line depicts the linear regression model and the surrounding grey-colored area represents the 95% confidence interval.

Article Snippet: To detect neutralizing antibodies using an immunoassay method, the cPass sVNT kit (GenScript, Piscataway, NJ, USA) was used. sVNT measures the inhibition of interactions between the horseradish peroxidase (HRP)-conjugated SARS-CoV-2 spike protein receptor-binding domain (RBD) and the extracellular domain of the human angiotensin-converting enzyme 2 (hACE2) receptor ( ).

Techniques: